Journal: Cell Reports Medicine

Article Title: Non-orthosteric inhibition of enolase 1 impedes growth of triple-negative breast cancer

doi: 10.1016/j.xcrm.2025.102451

Figure Lengend Snippet: SU212 inhibits ENO1 cellular localization and TNBC migration and metastasis (A–C) SU212 inhibits ENO1 cellular localization. MDA-MB-231 (top row) and EMT6 (bottom row) cells were treated with DMSO and SU212 (0.1 μM) for 6 h, and cells were fractionated for different cellular compartments. SDS-PAGE and western blot analysis were performed to identify ENO1 protein. Membranes were stripped and re-probed with respective loading control antibodies to ensure equal protein loading. (A) Cell membrane and cytosolic protein fractionation. β-catenin identifies the membrane fraction. (B) Cell nucleus and cytosolic protein fractionation. Lamin B1 identifies the nuclear fraction. (C) Mitochondrial and cytosolic protein fractionation. COX4 identifies the mitochondrial fraction. (D) MG132 and 3MA co-treatment partially reverses the SU212-associated inhibition of ENO1 cell membrane localization. (E) Matrigel dot invasion assay demonstrates SU212 treatment inhibits cell invasion and migration in TNBC cells. The data shown are mean ± SD ( n = 3). Data were analyzed using the Student’s t test. Numbers indicate a p value that is different compared with vehicle control. (F–H) SU212 treatment inhibits lung metastasis. In total, 1 × 10 5 EMT6 cells were implanted orthotopically in female NSG mice. Mice were randomized and divided into two groups. Each group was treated with either vehicle only or SU212 (30 mg/kg) for 21 days. Lungs were collected and processed for H&E staining. (F) Representative lung images collected at experiment end. (G) Representative images of H&E-stained lungs. Scale bar, 1,000 μm. (H) Number of metastatic foci. Data are shown as the mean ± SD ( n = 7/group). Numbers indicate the p value compared with vehicle control; data analyzed using Student’s t test.

Article Snippet: The following primary and secondary antibodies were used: ENO1 (Abcam, # ab227978; 1:10000), ENO3 (Novus Biologicals, # NBP1-31764; 1:2000) β-actin (CST, #4970S; 1:10000), GAPDH (CST, #2118S; 1:10000), β-catenin (CST, #8480T; 1:1000), Lamin B1 (CST, #13435S; 1:1000), COX4 (CST, #38563; 1:1000), anti-mouse IgG HRP-linked antibody (CST, #7076, 1:5000), and anti-rabbit IgG HRP-linked antibody (CST, #7074, 1:5000).

Techniques: Migration, SDS Page, Western Blot, Control, Membrane, Fractionation, Inhibition, Invasion Assay, Staining

Journal: eLife

Article Title: Tissue-specific mitochondrial HIGD1C promotes oxygen sensitivity in carotid body chemoreceptors

doi: 10.7554/eLife.78915

Figure Lengend Snippet: Reagents and resources.

Article Snippet: COX4I1 -Myc-FLAG in pCMV6-Entry , OriGene , RC209374.

Techniques: Sequencing, In Situ Hybridization, Plasmid Preparation